CLOSTRIDIUM DIFFICILE TOXIN B

Stock Code: 3574536
Manufacturer Part No: SML1153-2UG

Order Now for 7 day delivery

Descriptions

Biochem/physiol Actions

Clostridium difficile Toxin A and B, cation-dependent UDP-glucose glucosyltransferases, are cellular toxins that inactivate Rho (and Rho family small GTPases) through monoglucosylation of these family members. Effects of this monoglucosylation include disregulation of the actin cytoskeleton, cell rounding, cytotoxicity, and altered cellular signaling. Rho proteins are monoglucosylated by Toxin A and B using UDP-glucose as a cosubstrate. Rho, Rac and Cdc42 are included in the Rho subfamilies targeted by both toxins. Low molecular mass GTP-binding proteins that are not modified by Toxin A and B include Ras, Rab, Arf, or Ran subfamilies as well as heterotrimeric G proteins.

Inactivates Rho (and Rho family small GTPases). Causes disregulation of the actin cytoskeleton, cell rounding, cytotoxicity, and altered cellular signaling. Clostridium difficile is a bacteria that causes antibiotic-associated pseudomembranous colitis. This bacterium produces two high molecular weight exotoxins, toxin A and B. Toxin B is more effective than toxin A in disrupting human colonic epithelium in vitro.

Toxin B is 100-1,000-fold more cytotoxic than toxin A in inducing rounding-up of cells and destruction of the actin cytoskeleton.

Features and Benefits

This compound is featured on the GTP Binding Proteins (Low Molecular Weight) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

General description

Native toxin B purified from C. difficile strain VPI10463 (toxinotype 0)

Other Notes

After reconstitution, the reconstituted liquid can be frozen at -80 °C to -20 °C to extend stability to 1 year; however every freeze/thaw cycle wil cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations.Mixing: Swirl or pipette gently to mix. Vortexing may promote aggregation.

Reconstitution

Contents of the 50 µg SKU when reconstituted in 250 µL sterile distilled water will contain Toxin B at a concentration of approximately 0.2 mg/mL in 0.05?M Hepes, 0.15?M sodium chloride and 5% sucrose.Contents of the 2 µg SKU when reconstituted in 100 µL sterile distilled water will contain Toxin B at a concentration of approximately 0.02 mg/mL in 0.05?M Hepes, 0.15?M sodium chloride and 5% sucrose.