CLOSTRIDIUM DIFFICILE TOXIN A

CLOSTRIDIUM DIFFICILE TOXIN A

Biochem/physiol Actions

Clostridium difficile Toxin A and B, cation-dependent UDP-glucose glucosyltransferases, are cellular toxins that inactivate Rho (and Rho family small GTPases) through monoglucosylation of these family members. TcdA elicits effects primarily within the intestinal epithelium. Effects of this monoglucosylation include disregulation of the actin cytoskeleton, cell rounding, cytotoxicity, and altered cellular signaling. Rho proteins are monoglucosylated by Toxin A and B using UDP-glucose as a cosubstrate. Rho, Rac and Cdc42 are included in the Rho subfamilies targeted by both toxins. Low molecular mass GTP-binding proteins that are not modified by Toxin A and B include Ras, Rab, Arf, or Ran subfamilies as well as heterotrimeric G proteins.

General description

Native toxin A purified from C. difficile strain VPI10463 (toxinotype 0)

Other Notes

After reconstitution, the reconstituted liquid can be frozen at -80 °C to -20 °C to extend stability to 1 year; however every freeze/thaw cycle wil cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations.Mixing: Swirl or pipette gently to mix. Vortexing may promote aggregation.

Reconstitution

Contents of the 50UG sku when reconstituted in 250 µL sterile distilled water will contain Toxin B at a concentration of approximately 0.2 mg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.Contents of the 2UG sku when reconstituted in 250 µL sterile distilled water will contain Toxin B at a concentration of approximately 8 µg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.

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Code Description Quality Level Manufacturer Storage Temp. Form Assay Price Quantity
3574538 CLOSTRIDIUM DIFFICILE TOXIN A 200 SIGMA-ALDRICH 2-8°C lyophilized powder ≥95% (SDS-PAGE)
£576.64 (exc VAT) per 2UG
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3574539 CLOSTRIDIUM DIFFICILE TOXIN A 200 SIGMA-ALDRICH 2-8°C lyophilized powder ≥95% (SDS-PAGE)
£841.29 (exc VAT) per 50UG
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