Protein G from Streptococcus sp., recombinant, expressed in E. coli
Application
Protein G? from Streptococcus sp. has been used in in vitro actin labeling assay.
Biochem/physiol Actions
Protein G can bind all the human and mouse IgG subclasses. It can bind to both the fragment crystallizable (Fc) and antigen-binding fragment (Fab) components of the antibody.
General description
Protein G, a cell wall protein, is obtained from group G streptococci. The extracellular part of this protein is made of two/three small domains or serum albumin binding (GA domains) and two/three immunoglobulin (IgG) binding domains (B domains).
Genetically engineered truncated protein G; retains affinity for IgG, but lacks albumin and Fab binding sites and membrane-binding regions.
Physical form
lyophilized powder in a Tris-HCl buffer, pH 7.5
| Capacity | ~5 mg/mg, solid binding capacity (IgG) |
|---|---|
| Quality Level | 100 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Form | lyophilized solid |
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