Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Caution

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Packaging

1, 5 mg in glass bottle

Preparation Note

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

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Code Description Quality Level Manufacturer Storage Temp. Impurities Solubility Form Price Quantity
3589875 Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder 200 SIGMA-ALDRICH 2-8°C HIV, hepatitis B and hepatitis C, none detected aqueous acid: soluble powder
£96.75 (exc VAT) per 1MG
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3589876 Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder 200 SIGMA-ALDRICH 2-8°C HIV, hepatitis B and hepatitis C, none detected aqueous acid: soluble powder
£358.75 (exc VAT) per 5MG
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