Lipoxidase from Glycine max (soybean), Type V, ammonium sulfate suspension, 500,000-1,000,000 units/

Stock Code: 3591667
Manufacturer Part No: L6632-5MU
Order Now for 7 day delivery
£596.14 (exc VAT) per EACH
Quantity: - +

Analysis Note

Protein determined by biuret.

Application

Lipoxidase from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Physical form

Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0

Preparation Note

Prepared by ion exchange chromatography and hydrophobic interaction chromatography.

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH?9.0 at 25?°C when linoleic acid is the substrate in 3.0?ml volume (1?cm light path). One A234 unit is equivalent to the oxidation of 0.12 µmole of linoleic acid.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

There are no downloads for this product.