Gly-Gln monohydrate, >=97% (TLC)

Stock Code: 3591333
Manufacturer Part No: G5149-1G
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Amino Acid Sequence

Gly-Gln

Application

Heat-stable substitute for glutamine in mammalian cell culture media.

Biochem/physiol Actions

Gly-Gln monohydrate has been used for the preparation of glutamine solution in HTF (human tubal fluid) medium to assess the accumulation of ammonium in commercially available culture media and protein supplements for in vitro development of human pre-implantation embryos, when stored at 2–8°C and incubated at 37°C.

General description

Glycyl-glutamine (gly-gln) is a dipeptide that is used as a substitute for glutamine in cell culture. Higher cell yields are obtained when this dipeptide is used instead of glutamine, where gly-gln affects substrate utilization and metabolism. Cell growth is higher as the concentrations of this dipeptide remain high, due to lower peptidase affinity.

Packaging

1, 5 g in glass bottle

Quality Level300
InChI keyKLFWVRQACSYLOH-WCCKRBBISA-N
InChI1S/C7H13N3O4.H2O/c8-3-6(12)10-4(7(13)14)1-2-5(9)11;/h4H,1-3,8H2,(H2,9,11)(H,10,12)(H,13,14);1H2/t4-;/m0./s1
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Assay≥97% (HPLC)

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