Phosphatase, Alkaline from calf intestinal mucosa, for enzyme immunoassay, solution (clear, colorles

Stock Code: 3578885
Manufacturer Part No: 79390-1MG
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£246.54 (exc VAT) per 1MG
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Application


Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.


Biochem/physiol Actions


Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.


Other Notes


Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase


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Reconstitution


The solution can be used without dialysis (for enzyme immuno-assay).


Unit Definition


1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37?°C

pH~7.6
ManufacturerSIGMA-ALDRICH
Technique(s)enzyme immunoassay: suitable
Storage Temp.2-8°C
Impurities30 mM triethanolamine, 3 M NaCl, 0.1 mM ZnCl2, 1 mM MgCl2
Formsolution (clear, colorless)

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