Lactic Dehydrogenase, recombinant from E. coli, >=90 U/mg

Lactic Dehydrogenase, recombinant from E. coli, >=90 U/mg

Application

Lactic Dehydrogenase, recombinant fromE. coli has been used:in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assaysto prepare assay buffer to measure pyruvate kinase (PYK) by coupled assayin in vitro DltC D-alanylation assay

Biochem/physiol Actions

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.

General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

Unit Definition

One unit corresponds to the amount of enzyme which reduces 1 µmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3578844 Lactic Dehydrogenase, recombinant from E. coli, >=90 U/mg 100 SIGMA-ALDRICH −20°C powder
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3578845 Lactic Dehydrogenase, recombinant from E. coli, >=90 U/mg 100 SIGMA-ALDRICH −20°C powder
Click Add to Quote for price and delivery
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