Acylase I from porcine kidney, Grade I, lyophilized powder, >=2,000 units/mg protein

Analysis Note

Protein determined by biuret.

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .

Packaging

100, 500 mg in poly bottle

1, 5 g in poly bottle

Unit Definition

One unit will hydrolyze 1.0 µmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Composition Storage Temp. Form Price Quantity
3578939 Acylase I from porcine kidney, Grade I, lyophilized powder, >=2,000 units/mg protein 200 SIGMA-ALDRICH Protein, ≥60% −20°C lyophilized powder
£126.05 (exc VAT) per 100MG
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3578940 Acylase I from porcine kidney, Grade I, lyophilized powder, >=2,000 units/mg protein 200 SIGMA-ALDRICH Protein, ≥60% −20°C lyophilized powder
£739.61 (exc VAT) per 1G
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3578941 Acylase I from porcine kidney, Grade I, lyophilized powder, >=2,000 units/mg protein 200 SIGMA-ALDRICH Protein, ≥60% −20°C lyophilized powder
£417.86 (exc VAT) per 500MG
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3578942 Acylase I from porcine kidney, Grade I, lyophilized powder, >=2,000 units/mg protein 200 SIGMA-ALDRICH Protein, ≥60% −20°C lyophilized powder
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