beta-Amylase from barley, Type II-B, 20-80 units/mg protein (biuret)

Descriptions

Application

? -Amylase is used to hydrolyze ? bonds of ?-linked polysaccharides, such as starch and glycogen. ? -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides . ? -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity .

Biochem/physiol Actions

?-Amylase hydrolyzes the ?-(1,4) glucan linkages in polysaccharides of three or more ?-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline ?-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.

General description

We are committed to bringing you?Greener?Alternative Products, which adhere to one or more of The 12 Principles of?Greener?Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

10000, 50000, 250000 units in poly bottle

Quality

Crude

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH?4.8 at 20 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579028	beta-Amylase from barley, Type II-B, 20-80 units/mg protein (biuret)	200	SIGMA-ALDRICH	2-8°C	powder	£102.10 (exc VAT) per EACH	- +
3579029	beta-Amylase from barley, Type II-B, 20-80 units/mg protein (biuret)	200	SIGMA-ALDRICH	2-8°C	powder	Click Add to Quote for price and delivery	- +
3579030	beta-Amylase from barley, Type II-B, 20-80 units/mg protein (biuret)	200	SIGMA-ALDRICH	2-8°C	powder	£408.11 (exc VAT) per EACH	- +