Carboxypeptidase G from Pseudomonas sp., lyophilized powder, >=3 units/mg protein

Carboxypeptidase G from Pseudomonas sp., lyophilized powder, >=3 units/mg protein

Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO?cGMP signaling in lung adenocarcinoma cells.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves ?-glutamyl pteroyl poly-?-glutamate yielding pteroyl-?-glutamate (folic acid) and free glutamate. It is considered highly specific for the ?-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Packaging

5 units in poly bottle

20 units in glass bottle

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified

Solutions should be prepared fresh prior to use.

Unit Definition

One unit will hydrolyze 1.0 µmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Composition Storage Temp. Solubility Form Price Quantity
3579314 Carboxypeptidase G from Pseudomonas sp., lyophilized powder, >=3 units/mg protein 300 SIGMA-ALDRICH Protein, ~70% biuret −20°C H2O: soluble 0.5 mg/mL lyophilized powder
£1,195.07 (exc VAT) per EACH
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3579315 Carboxypeptidase G from Pseudomonas sp., lyophilized powder, >=3 units/mg protein 300 SIGMA-ALDRICH Protein, ~70% biuret −20°C H2O: soluble 0.5 mg/mL lyophilized powder
£395.57 (exc VAT) per EACH
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+