beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein

beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein

Application

?-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA). ?-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter. It may be used as a positive control protein with anti-?-galactosidase antibodies.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

General description

?-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Packaging

1000, 3000, 5000, 15000 units in serum bottle

Physical form

Contains Tris buffer salts and magnesium chloride

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Composition Storage Temp. Form Price Quantity
3579558 beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein 300 SIGMA-ALDRICH Protein, ≥60% biuret −20°C lyophilized powder
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3579559 beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein 300 SIGMA-ALDRICH Protein, ≥60% biuret −20°C lyophilized powder
£133.85 (exc VAT) per EACH
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3579560 beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein 300 SIGMA-ALDRICH Protein, ≥60% biuret −20°C lyophilized powder
£302.25 (exc VAT) per EACH
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3579561 beta-Galactosidase from Escherichia coli, Grade VIII, lyophilized powder, >=500 units/mg protein 300 SIGMA-ALDRICH Protein, ≥60% biuret −20°C lyophilized powder
£481.93 (exc VAT) per EACH
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