alpha-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder

Descriptions

Application

?-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies. It has also been used in the hydrolysis of ?-gliadins prior to mass spectroscopy studies.

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m ?, ?, ?, ? and ? chymotrypsin (depending upon the conditions of activation). Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.

General description

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97.

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5 g in poly bottle

100, 250 mg in poly bottle

Unit Definition

After activation to Chymotrypsin, one unit will hydrolyze 1.0 µmole of BTEE per min at pH 7.8 at 25 °C.

Specifications

No detailed specifications are available for this product.

Downloads

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Code	Description	Quality Level	Manufacturer	Solubility	Form	Price	Quantity
3579208	alpha-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder	200	SIGMA-ALDRICH	1 mM HCl: soluble 10 mg/mL, clear, colorless	essentially salt-free, lyophilized powder	£48.33 (exc VAT) per 100MG	- +
3579209	alpha-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder	200	SIGMA-ALDRICH	1 mM HCl: soluble 10 mg/mL, clear, colorless	essentially salt-free, lyophilized powder	£200.57 (exc VAT) per 1G	- +
3579210	alpha-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder	200	SIGMA-ALDRICH	1 mM HCl: soluble 10 mg/mL, clear, colorless	essentially salt-free, lyophilized powder	£80.65 (exc VAT) per 250MG	- +
3579211	alpha-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder	200	SIGMA-ALDRICH	1 mM HCl: soluble 10 mg/mL, clear, colorless	essentially salt-free, lyophilized powder	£969.43 (exc VAT) per 5G	- +