Heparinase I from Flavobacterium heparinum, Lyophilized powder stabilized with approx. 25% bovine se

Application

Heparinase I from Flavobacterium heparinum has been used:in the digestion of plasma samples to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumoniain enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells

Biochem/physiol Actions

Heparinase I from Flavobacterium heparinum acts by cleaving the ?-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein?s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit (Sigma unit) will form 0.1 µmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

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