Endoproteinase Glu-C from Staphylococcus aureus V8, Type XVII-B, lyophilized powder, 500-1,000 units

Endoproteinase Glu-C from Staphylococcus aureus V8, Type XVII-B, lyophilized powder, 500-1,000 units

Application

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments.

It is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.

Biochem/physiol Actions

Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

Unit Definition

One unit will hydrolyze 1 ?mole of N-t-Boc-L-glutamic acid ?-phenyl ester per min at pH 7.8 at 37°C. One unit is equivalent to ~0.004 casein digestion unit.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3581024 Endoproteinase Glu-C from Staphylococcus aureus V8, Type XVII-B, lyophilized powder, 500-1,000 units 200 SIGMA-ALDRICH −20°C lyophilized powder
£59.34 (exc VAT) per EACH
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3581025 Endoproteinase Glu-C from Staphylococcus aureus V8, Type XVII-B, lyophilized powder, 500-1,000 units 200 SIGMA-ALDRICH −20°C lyophilized powder
£894.21 (exc VAT) per EACH
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3581026 Endoproteinase Glu-C from Staphylococcus aureus V8, Type XVII-B, lyophilized powder, 500-1,000 units 200 SIGMA-ALDRICH −20°C lyophilized powder
£190.82 (exc VAT) per EACH
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