Luciferase from Photinus pyralis (firefly), recombinant, expressed in E. coli, buffered aqueous solu

Luciferase from Photinus pyralis (firefly), recombinant, expressed in E. coli, buffered aqueous solu

Application

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

General description

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Physical form

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Unit Definition

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 µl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 µCi of 14C in a PPO/POPOP cocktail.

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Code Description Quality Level Manufacturer Storage Temp. Form Assay Price Quantity
3579853 Luciferase from Photinus pyralis (firefly), recombinant, expressed in E. coli, buffered aqueous solu 200 SIGMA-ALDRICH −20°C buffered aqueous solution ≥98% (SDS-PAGE)
£164.36 (exc VAT) per 1MG
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3579854 Luciferase from Photinus pyralis (firefly), recombinant, expressed in E. coli, buffered aqueous solu 200 SIGMA-ALDRICH −20°C buffered aqueous solution ≥98% (SDS-PAGE)
£664.39 (exc VAT) per EACH
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