L-Glutamic Dehydrogenase (NADP) from Proteus sp., buffered aqueous solution, >=400 units/mg protein

L-Glutamic Dehydrogenase (NADP) from Proteus sp., buffered aqueous solution, >=400 units/mg protein

Application

This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. In vitro, various activity assays of this enzyme examine the conversion of α-ketoglutarate to L-glutamate, in the presence of excess ammonium ions (NH4+) and NADPH.

Biochem/physiol Actions

L-glutamic dehydrogenase catalyzes the conversion of glutamate to ?-ketoglutarate.

Other Notes

Note: Do not confuse with non-specific L-GLDH, EC 1.4.1.3.

Physical form

Solution in 50 mM Tris HCl, pH 7.8, 5 mM Na2EDTA containing 0.05% sodium azide

Physical properties

Isoelectric point : 4.6Michaelis constants : 1.1 X 10-3M (NH3), 3.4 X 10-4M (α-Ketoglutarate)1.2 X 10-3M (L-Glutamate), 1.4 X 10-5M (NADPH), 1.5 X 10-5M (NADP+)Structure : 6 subunits (M.W.50,000) per mol of enzymeInhibitors : Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine,2,2′-dithiopyridineOptimum pH : 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG) Optimum temperature : 45oC(α-KG−L-Glu) 45-55oC (L-Glu→α-KG) pH stability : pH 6.0 - 8.5 (25oC, 20hr) Thermal stability : below 50oC (pH 7.4, 10min)

Unit Definition

One unit will reduce 1.0?µmole of α-ketoglutarate to L-glutamate per min at pH?8.3 at 30?°C in the presence of ammonium ions and NADPH.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579543 L-Glutamic Dehydrogenase (NADP) from Proteus sp., buffered aqueous solution, >=400 units/mg protein 200 SIGMA-ALDRICH 2-8°C buffered aqueous solution
£289.71 (exc VAT) per EACH
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3579544 L-Glutamic Dehydrogenase (NADP) from Proteus sp., buffered aqueous solution, >=400 units/mg protein 200 SIGMA-ALDRICH 2-8°C buffered aqueous solution
£1,101.75 (exc VAT) per EACH
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+