Lysozyme from chicken egg white, BioUltra, lyophilized powder, >=98% (SDS-PAGE), >=40,000 units/mg p

Lysozyme from chicken egg white, BioUltra, lyophilized powder, >=98% (SDS-PAGE), >=40,000 units/mg p

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Lysozyme from chicken egg white has been used: in dielectric spectroscopystudies of dynamics of protein as a control to measure the human lysozyme activity as a supplement in soaking solutionto treat lenses

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Features and Benefits

Highly purified by repeated crystallization and dialysis Each lot is use-tested for isolation of plasmid DNA from E. coli

General description

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Packaging

1, 5 g in poly bottle

500 mg in poly bottle

Preparation Note

3× crystallized

Unit Definition

One unit will produce a δA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

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Code Description Quality Level Manufacturer Composition Product Line Form Assay Price Quantity
3579817 Lysozyme from chicken egg white, BioUltra, lyophilized powder, >=98% (SDS-PAGE), >=40,000 units/mg p 200 SIGMA-ALDRICH Protein, ≥90% BioUltra lyophilized powder ≥98% (SDS-PAGE)
£168.54 (exc VAT) per 1G
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3579818 Lysozyme from chicken egg white, BioUltra, lyophilized powder, >=98% (SDS-PAGE), >=40,000 units/mg p 200 SIGMA-ALDRICH Protein, ≥90% BioUltra lyophilized powder ≥98% (SDS-PAGE)
£78.28 (exc VAT) per 500MG
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3579819 Lysozyme from chicken egg white, BioUltra, lyophilized powder, >=98% (SDS-PAGE), >=40,000 units/mg p 200 SIGMA-ALDRICH Protein, ≥90% BioUltra lyophilized powder ≥98% (SDS-PAGE)
£557.14 (exc VAT) per 5G
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