Phosphatase, Alkaline from calf intestinal mucosa, for enzyme immunoassay, solution (clear, colorles
Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Other Notes
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
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Reconstitution
The solution can be used without dialysis (for enzyme immuno-assay).
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37?°C
| pH | ~7.6 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Technique(s) | enzyme immunoassay: suitable |
| Storage Temp. | 2-8°C |
| Impurities | 30 mM triethanolamine, 3 M NaCl, 0.1 mM ZnCl2, 1 mM MgCl2 |
| Form | solution (clear, colorless) |
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