Choloylglycine Hydrolase from Clostridium perfringens (C.?ßwelchii), lyophilized powder, >=100

Descriptions

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Packaging

3000, 7500 units in poly bottle

1500 units in glass bottle

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Unit Definition

One unit will hydrolyze 1.0 µmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Storage Temp.	Form	Price	Quantity
3579190	Choloylglycine Hydrolase from Clostridium perfringens (C.?ßwelchii), lyophilized powder, >=100	200	SIGMA-ALDRICH	Protein, ≥50% biuret	−20°C	lyophilized powder	£385.82 (exc VAT) per EACH	- +
3579191	Choloylglycine Hydrolase from Clostridium perfringens (C.?ßwelchii), lyophilized powder, >=100	200	SIGMA-ALDRICH	Protein, ≥50% biuret	−20°C	lyophilized powder	£668.57 (exc VAT) per EACH	- +
3579192	Choloylglycine Hydrolase from Clostridium perfringens (C.?ßwelchii), lyophilized powder, >=100	200	SIGMA-ALDRICH	Protein, ≥50% biuret	−20°C	lyophilized powder	Click Add to Quote for price and delivery	- +