L-GLUTAMATE OXIDASE FROM STREPTOMYCES&

Application

L-Glutamate Oxidase from Streptomyces sp may be used:as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor developmentas a component of pencil graphite electrode for biosensing L-glutamate

Biochem/physiol Actions

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to ?-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

General description

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

This product has a 6xHistidine tag with a thrombin cleavage site.

Unit Definition

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579495 L-GLUTAMATE OXIDASE FROM STREPTOMYCES& 200 SIGMA-ALDRICH −20°C solid
£161.57 (exc VAT) per EACH
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3579496 L-GLUTAMATE OXIDASE FROM STREPTOMYCES& 200 SIGMA-ALDRICH −20°C solid
£523.71 (exc VAT) per EACH
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