Achromopeptidase from bacteria, partially purified powder, >=20,000 units/mg solid

Descriptions

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Achromopeptidase from bacteria may be used:in the homogenization step for genomic DNA extraction from microbial biofilmin the lysis of gram-positive strains(for DNA extraction from Nocardia strains

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 µn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀= 0.6 over 2 hours at 37 °C.

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Physical form

Partially purified powder.

Unit Definition

One unit will produce a change in A₆₀₀of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price		Quantity
3578956	Achromopeptidase from bacteria, partially purified powder, >=20,000 units/mg solid	200	SIGMA-ALDRICH	−20°C	partially purified powder	£702.00 (exc VAT) per EACH		- +
3578957	Achromopeptidase from bacteria, partially purified powder, >=20,000 units/mg solid	200	SIGMA-ALDRICH	−20°C	partially purified powder	Click Add to Quote for price and delivery		- +