Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder

Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder

Analysis Note

One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.

Application

Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Components

The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

Packaging

100, 250, 500 mg in glass bottle

1, 5 g in glass bottle

25 mg in poly bottle

Preparation Note

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Unit Definition

One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

One trypsin unit will produce a δA253 of 0.001 per min with BAEE as substrate at pH?7.6 at 25?°C; reaction volume 3.2?ml, 1?cm light path.

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Code Description Quality Level Manufacturer Storage Temp. Solubility Form Price Quantity
3581487 Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder 300 SIGMA-ALDRICH 2-8°C phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), H2O: >10 mg/mL, soluble lyophilized powder
£238.18 (exc VAT) per 100MG
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3581488 Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder 300 SIGMA-ALDRICH 2-8°C phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), H2O: >10 mg/mL, soluble lyophilized powder
£1,168.61 (exc VAT) per 1G
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3581489 Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder 300 SIGMA-ALDRICH 2-8°C phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), H2O: >10 mg/mL, soluble lyophilized powder
£410.89 (exc VAT) per 250MG
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3581490 Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder 300 SIGMA-ALDRICH 2-8°C phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), H2O: >10 mg/mL, soluble lyophilized powder
£81.20 (exc VAT) per 25MG
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3581491 Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder 300 SIGMA-ALDRICH 2-8°C phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), H2O: >10 mg/mL, soluble lyophilized powder
£795.32 (exc VAT) per 500MG
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3581492 Trypsin inhibitor from Glycine max (soybean), Type I-S, lyophilized powder 300 SIGMA-ALDRICH 2-8°C phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), H2O: >10 mg/mL, soluble lyophilized powder
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