Phosphatase, Alkaline from calf intestinal mucosa, for enzyme immunoassay, solution (clear, colorles

Phosphatase, Alkaline from calf intestinal mucosa, for enzyme immunoassay, solution (clear, colorles

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Other Notes

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

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Reconstitution

The solution can be used without dialysis (for enzyme immuno-assay).

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37?°C

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Code Description pH Manufacturer Technique(s) Storage Temp. Impurities Form Price Quantity
3578885 Phosphatase, Alkaline from calf intestinal mucosa, for enzyme immunoassay, solution (clear, colorles ~7.6 SIGMA-ALDRICH enzyme immunoassay: suitable 2-8°C 30 mM triethanolamine, 3 M NaCl, 0.1 mM ZnCl2, 1 mM MgCl2 solution (clear, colorless)
£246.54 (exc VAT) per 1MG
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3578886 Phosphatase, Alkaline from calf intestinal mucosa, for enzyme immunoassay, solution (clear, colorles ~7.6 SIGMA-ALDRICH enzyme immunoassay: suitable 2-8°C 30 mM triethanolamine, 3 M NaCl, 0.1 mM ZnCl2, 1 mM MgCl2 solution (clear, colorless)
£913.71 (exc VAT) per 5MG
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