Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver, ammonium sulfate suspension, 1

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver, ammonium sulfate suspension, 1

Application

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Biochem/physiol Actions

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

General description

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Physical form

Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0

Unit Definition

One unit will convert 1.0 µmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579876 Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver, ammonium sulfate suspension, 1 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
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3579877 Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver, ammonium sulfate suspension, 1 200 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£424.82 (exc VAT) per EACH
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