Carboxypeptidase A from bovine pancreas, (Type II-PMSF treated), >=50 units/mg protein, aqueous susp

Descriptions

Analysis Note

Protein determined by E1%/278

Application

The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate ?-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.

Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.

Packaging

500, 2500, 5000 units in glass bottle

Preparation Note

Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.

Unit Definition

One unit will hydrolyze 1.0 µmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Impurities	Form	Price	Quantity
3579302	Carboxypeptidase A from bovine pancreas, (Type II-PMSF treated), >=50 units/mg protein, aqueous susp	300	SIGMA-ALDRICH	2-8°C	≤0.05 BTEE units/mg protein chymotrypsin, ≤10 BAEE units/mg protein trypsin	aqueous suspension	£307.82 (exc VAT) per EACH	- +
3579303	Carboxypeptidase A from bovine pancreas, (Type II-PMSF treated), >=50 units/mg protein, aqueous susp	300	SIGMA-ALDRICH	2-8°C	≤0.05 BTEE units/mg protein chymotrypsin, ≤10 BAEE units/mg protein trypsin	aqueous suspension	£161.57 (exc VAT) per EACH	- +
3579304	Carboxypeptidase A from bovine pancreas, (Type II-PMSF treated), >=50 units/mg protein, aqueous susp	300	SIGMA-ALDRICH	2-8°C	≤0.05 BTEE units/mg protein chymotrypsin, ≤10 BAEE units/mg protein trypsin	aqueous suspension	£566.89 (exc VAT) per EACH	- +