Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified)

Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified)

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (~10) and another with a low ratio (~1).Reaction: ATP → ADP+Pi → AMP+2Pi.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Other Notes

Mixture of high and low ratio isoenzymes.

Packaging

100, 200 units in glass bottle

Sold on the basis of ATPase units

500, 1000, 2000, 5000 units in poly bottle

Quality

Contains ≤ 5% acid phosphatase.

Unit Definition

One unit will liberate 1.0 µmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

No detailed specifications are available for this product.

There are no downloads for this product.

Code Description Quality Level Manufacturer Composition Form Price Quantity
3579044 Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified) 200 SIGMA-ALDRICH Protein, 30-60% lyophilized powder (partially purified)
Click Add to Quote for price and delivery
-
+
3579045 Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified) 200 SIGMA-ALDRICH Protein, 30-60% lyophilized powder (partially purified)
Click Add to Quote for price and delivery
-
+
3579046 Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified) 200 SIGMA-ALDRICH Protein, 30-60% lyophilized powder (partially purified)
Click Add to Quote for price and delivery
-
+
3579047 Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified) 200 SIGMA-ALDRICH Protein, 30-60% lyophilized powder (partially purified)
Click Add to Quote for price and delivery
-
+
3579048 Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified) 200 SIGMA-ALDRICH Protein, 30-60% lyophilized powder (partially purified)
Click Add to Quote for price and delivery
-
+
3579049 Apyrase from potatoes, ATPase 40-200 units/mg protein, lyophilized powder (partially purified) 200 SIGMA-ALDRICH Protein, 30-60% lyophilized powder (partially purified)
Click Add to Quote for price and delivery
-
+