Deoxyribonuclease II from porcine spleen, Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg p

Descriptions

Application

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

DNase II from Sigma has been used to treat transformed cells during the purification of ?-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3?-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked ?·? heterodimer of approximately 45,000 Da molecular mass.

Packaging

20000, 80000 units in poly bottle

Physical form

Contains sodium chloride

Unit Definition

One Kunitz unit will produce a δA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Storage Temp.	Form	Price		Quantity
3579338	Deoxyribonuclease II from porcine spleen, Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg p	200	SIGMA-ALDRICH	Protein, 60-80%	−20°C	lyophilized powder	£68.25 (exc VAT) per EACH		- +
3579339	Deoxyribonuclease II from porcine spleen, Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg p	200	SIGMA-ALDRICH	Protein, 60-80%	−20°C	lyophilized powder	£208.93 (exc VAT) per EACH		- +