Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffe

Descriptions

Application

Thymidine phosphorylase has been used in a study to assess TAS-102 treatment in patients with advanced solid tumors. Thymidine phosphorylase has also been used in a study to investigate antitumor effects of the FP3 protein on patient-derived tumor tissue xenograft models of primary colon carcinoma.

Biochem/physiol Actions

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

General description

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

Packaging

5000 units in glass bottle

Physical form

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

Preparation Note

Cloned from E. coli and produced in overexpressing E. coli

Unit Definition

One unit will convert 1.0 µmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3581413	Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffe	300	SIGMA-ALDRICH	2-8°C	buffered aqueous solution	£229.82 (exc VAT) per EACH	- +
3581414	Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffe	300	SIGMA-ALDRICH	2-8°C	buffered aqueous solution	£196.39 (exc VAT) per EACH	- +
3581415	Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffe	300	SIGMA-ALDRICH	2-8°C	buffered aqueous solution	£788.36 (exc VAT) per EACH	- +