Collagenase from Clostridium histolyticum, high purity, purified by chromatography, Type VII, 4-15 F

Descriptions

Application

Collagenase has been used in the preparation of arterial tissue for the study of advanced glycosylation end products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. Optimal pH ranges from 6.3-8.8. The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

Packaging

1500, 3000, 7500, 15000, 30000, 60000 units in glass bottle

Physical form

Lyophilized powder containing calcium chloride

Preparation Note

A stock solution may be prepared by dissolving 0.05 - 0.1 mg of collagenase in one mL of 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA), pH 7.4 at 37 °C.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Solubility	Form	Price	Quantity
3579102	Collagenase from Clostridium histolyticum, high purity, purified by chromatography, Type VII, 4-15 F	200	SIGMA-ALDRICH	Protein, ≥85% biuret	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	lyophilized powder	£128.75 (exc VAT) per EACH	- +
3579103	Collagenase from Clostridium histolyticum, high purity, purified by chromatography, Type VII, 4-15 F	200	SIGMA-ALDRICH	Protein, ≥85% biuret	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	lyophilized powder	£667.50 (exc VAT) per EACH	- +
3579104	Collagenase from Clostridium histolyticum, high purity, purified by chromatography, Type VII, 4-15 F	200	SIGMA-ALDRICH	Protein, ≥85% biuret	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	lyophilized powder	£215.00 (exc VAT) per EACH	- +
3579105	Collagenase from Clostridium histolyticum, high purity, purified by chromatography, Type VII, 4-15 F	200	SIGMA-ALDRICH	Protein, ≥85% biuret	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	lyophilized powder	Click Add to Quote for price and delivery	- +
3579106	Collagenase from Clostridium histolyticum, high purity, purified by chromatography, Type VII, 4-15 F	200	SIGMA-ALDRICH	Protein, ≥85% biuret	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	lyophilized powder	£466.25 (exc VAT) per EACH	- +