Sphingomyelinase from Bacillus cereus, buffered aqueous glycerol solution, >=100 units/mg protein (L
Application
Sphingomyelinase from Bacillus cereus has been used:in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell linesin lipoprotein modification for the generation of cholesterol crystals in vitroto aggregate LDL (low-density lipoprotein)
Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.
Biochem/physiol Actions
Sphingomyelin phosphodiesterase(SMPD) participates in the metabolic reactions comprisingsphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
General description
Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.
Physical form
Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.
Unit Definition
One unit will hydrolyze 1.0 µmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
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