?-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
General description
?-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for ?-lactam antibiotics. This enzyme is present in Gram-negative bacteria.
One unit will hydrolyze 1.0?µmole of benzylpenicillin per min at pH?7.0 at 25?°C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600?Levy or 75?Pollock units.