Diamine Oxidase from porcine kidney, >=0.05 unit/mg solid

Descriptions

Application

Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.

Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.

Biochem/physiol Actions

Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.

General description

Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.

Packaging

1, 5, 10 g in poly bottle

250 mg in poly bottle

Unit Definition

One unit will oxidize 1.0 µmole of putrescine per hr at pH 7.2 at 37 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Solubility	Form	Price	Quantity
3579363	Diamine Oxidase from porcine kidney, >=0.05 unit/mg solid	200	SIGMA-ALDRICH	−20°C	100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL	solid	Click Add to Quote for price and delivery	- +
3579364	Diamine Oxidase from porcine kidney, >=0.05 unit/mg solid	200	SIGMA-ALDRICH	−20°C	100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL	solid	£338.46 (exc VAT) per 1G	- +
3579365	Diamine Oxidase from porcine kidney, >=0.05 unit/mg solid	200	SIGMA-ALDRICH	−20°C	100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL	solid	£105.72 (exc VAT) per 250MG	- +
3579366	Diamine Oxidase from porcine kidney, >=0.05 unit/mg solid	200	SIGMA-ALDRICH	−20°C	100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL	solid	£1,149.11 (exc VAT) per 5G	- +