Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein

Descriptions

Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Cleavage of DNA by Nuclease micrococcal is greater at the 5? sides of A or T nucleotides than at G or C.

Other Notes

Note: One µmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a δA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37?°C in a 3.55 mL reaction volume. Light path = 1 cm.

Packaging

50, 200, 500 units in serum bottle

Unit Definition

One unit will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Storage Temp.	Form	Price	Quantity
3579921	Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein	300	SIGMA-ALDRICH	Protein, ≥40% E1%/280 (Balance primarily sodium citrate)	−20°C	lyophilized powder	£497.25 (exc VAT) per EACH	- +
3579922	Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein	300	SIGMA-ALDRICH	Protein, ≥40% E1%/280 (Balance primarily sodium citrate)	−20°C	lyophilized powder	£983.36 (exc VAT) per EACH	- +
3579923	Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein	300	SIGMA-ALDRICH	Protein, ≥40% E1%/280 (Balance primarily sodium citrate)	−20°C	lyophilized powder	£197.79 (exc VAT) per EACH	- +
3579926	Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein	200	SIGMA-ALDRICH	Protein, ≥40% E1%/280 (Balance primarily sodium acetate)	−20°C	powder	£409.50 (exc VAT) per EACH	- +
3579927	Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein	200	SIGMA-ALDRICH	Protein, ≥40% E1%/280 (Balance primarily sodium acetate)	−20°C	powder	£890.04 (exc VAT) per EACH	- +
3579928	Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein	200	SIGMA-ALDRICH	Protein, ≥40% E1%/280 (Balance primarily sodium acetate)	−20°C	powder	£165.75 (exc VAT) per EACH	- +