3-Phosphoglyceric Phosphokinase from bakers yeast (S. cerevisiae), ammonium sulfate suspension, >=10
Analysis Note
Protein determined by TCA Biuret.
Application
3-Phosphoglyceric Phosphokinase generates ATP by catalyzing the transfer of a phosphate group from 1,3-diphosphoglycerate to ADP. 3-Phosphoglycerate Phosphokinase is used to study glycolysis and gluconeogenesis. It has also been used to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase.
The enzyme has been used in the assay of glyceraldehyde-3-phosphate dehydrogenase.
Biochem/physiol Actions
3-Phosphoglyceric Phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate Phosphokinase activity is essential for glycolysis and gluconeogenesis.
Physical form
Crystalline suspension in 3.0 M (NH4)2SO4 and 0.04 M tetrasodium pyrophosphate solution, pH 8.0
Unit Definition
One unit will convert 1.0 µmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.
No detailed specifications are available for this product.
There are no downloads for this product.
