Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg

Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg

Stock Code: 3578879
Manufacturer Part No: 77151-1G
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£105.02 (exc VAT) per 1G
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Analysis Note


Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.


Application


Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.


Biochem/physiol Actions


Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin


Other Notes


Sales restrictions may apply


Unit Definition


1 U corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Cat.No. 51290, as substrate) 15′000 absorbance-U as described above are equivalent to ∼1 Bergmeyer-U. 1 Bergmeyer-U is the amount of enzyme which hydrolyzes 1 μmol Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37°C

Colourslightly beige
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formpowder

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