Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Application
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
Biochem/physiol Actions
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Other Notes
Sales restrictions may apply
Unit Definition
1 U corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Cat.No. 51290, as substrate) 15′000 absorbance-U as described above are equivalent to ∼1 Bergmeyer-U. 1 Bergmeyer-U is the amount of enzyme which hydrolyzes 1 μmol Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37°C
| Colour | slightly beige |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Form | powder |
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