Lyticase from Arthrobacter luteus, lyophilized powder, >=2,000 units/mg protein, Protein >=20 % by b

Lyticase from Arthrobacter luteus, lyophilized powder, >=2,000 units/mg protein, Protein >=20 % by b

Stock Code: 3579780
Manufacturer Part No: L2524-25KU
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Application


Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation.


Biochem/physiol Actions


Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.


Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest the cell walls of yeast and generate spheroplasts from fungi for transformation. It contains ?-(1?3)-glucan laminaripentaohydrolase along with ?-(1?3)-glucanase, protease, and mannanase activities. Lyticase is used for yeast cells like Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.


Other Notes


View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


Packaging


50000, 200000 units in poly bottle


10000, 25000 units in glass bottle


Physical form


Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers


Unit Definition


One unit will produce a δA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥20% biuret
Storage Temp.−20°C
Formlyophilized powder

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