beta-Glucuronidase from Helix pomatia, Type H-2, aqueous solution, >=85,000 units/mL
Application
New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Packaging
2 mL in glass bottle
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37?°C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37?°C.
| Quality Level | 300 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Form | aqueous solution |
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