Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein

Stock Code: 3579921
Manufacturer Part No: N3755-200UN
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Application


Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.


Biochem/physiol Actions


Hydrolyzes 5′-phosphodiester bonds of DNA.


Cleavage of DNA by Nuclease micrococcal is greater at the 5? sides of A or T nucleotides than at G or C.


Other Notes


Note: One µmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a δA260 of 1.0 in 30 min at pH 8.8 at 37?°C in a 3.55 mL reaction volume. Light path = 1 cm.


Packaging


50, 200, 500 units in serum bottle


Unit Definition


One unit will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥40% E1%/280 (Balance primarily sodium citrate)
Storage Temp.−20°C
Formlyophilized powder

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