Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein

Stock Code: 3579928
Manufacturer Part No: N5386-50UN
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Application


Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.


Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.


Biochem/physiol Actions


Hydrolyzes 5′-phosphodiester bonds of DNA.


Other Notes


Note: One µmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a δA260 of 1.0 in 30 min at pH 8.8 at 37?°C in a 3.55 mL reaction volume. Light path = 1 cm.


Packaging


50, 200, 500 units in glass bottle

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥40% E1%/280 (Balance primarily sodium acetate)
Storage Temp.−20°C
Formpowder

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