Trypsin Agarose, buffered aqueous suspension, trypsin from bovine pancreas

Stock Code: 3581406
Manufacturer Part No: T1763-50UN
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Application


A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography.


Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides.


General description


The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.


Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.


Other Notes


Insolubilized


Packaging


50 units in glass bottle


Physical form


Suspension in approx. 10 mM acetic acid, pH 3.2


Unit Definition


One unit will hydrolyze 1.0 µmole of BAEE per min at pH 8.0 at 30?°C (titrimetric assay).

Quality Level200
ManufacturerSIGMA-ALDRICH
Matrixcross-linked beaded agarose
Storage Temp.2-8°C
Formbuffered aqueous suspension

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