Transglutaminase from guinea pig liver, lyophilized powder, >=1.5 units/mg protein

Stock Code: 3581434
Manufacturer Part No: T5398-1UN
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Application


This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.


Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.


Biochem/physiol Actions


Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into ?-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of ?-glutamyl-?-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.


Physical form


Lyophilized powder containing Tris and dithioerythritol


Unit Definition


One unit will catalyze the formation of 1.0 µmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥80% modified Warburg-Christian
SolubilityH2O: soluble 1.0 mg/mL, clear
Formlyophilized powder

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