Thrombin from human plasma, lyophilized powder, Suitable for routine use in the thrombin time test

Thrombin from human plasma, lyophilized powder, Suitable for routine use in the thrombin time test

Stock Code: 3581484
Manufacturer Part No: T8885-10VL
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£286.36 (exc VAT) per EACH
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Analysis Note


The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.


Application


Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study that compared efficacy and safety of low-molecular-weight heparins (LMWHs) to other anticoagulants as venous thromboembolism prophylaxis in major orthopedic surgery.


Biochem/physiol Actions


Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.


General description


Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.


Other Notes


View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.


Reconstitution


When reconstituted with 1 ml H2O, one vial will contain 10 mg bovine serum albumin, 0.15 M NaCl and 0.05 M sodium citrate, pH 6.5


Unit Definition


Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Quality Level300
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
ImpuritiesHIV, hepatitis B and hepatitis C, tested negative
Formlyophilized powder

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