Thrombin from human plasma, lyophilized powder, >=2800 NIH units/mg protein (E1%/280, 18.3)

Thrombin from human plasma, lyophilized powder, >=2800 NIH units/mg protein (E1%/280, 18.3)

Stock Code: 3581397
Manufacturer Part No: T1063-1KU
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Analysis Note


The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.


Application


Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to evaluate the in vitro effect of low molecular weight heparin on thrombin generation in cirrhotic patients at different stages of liver disease.


Biochem/physiol Actions


Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.


General description


Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.


Other Notes


View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.


Physical form


Lyophilized from 0.02 M Bis/Tris buffer, pH 6.5, 0.15 M NaCl and 0.1% PEG-8000


Unit Definition


Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
ImpuritiesHIV, hepatitis B and hepatitis C, tested negative
Formlyophilized powder

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