Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.
General description
Sequential action of sulfatase from Helix pomatia and ?-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes ?-naphthyl sulfate much faster than ?-naphthyl sulfate.
Unit Definition
One unit will hydrolyze 1.0 µmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).