Sucrose Phosphorylase, recombinant, expressed in E. coli, lyophilized powder, >=45 units/mg solid

Stock Code: 3581290
Manufacturer Part No: S0937-1000UN

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Descriptions

Application

Sucrose Phosphorylase has been used in sucrose determination in wheat plant and in sucrose hydrogen production.

Sucrose phosphorylase has been used in a study to assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides. Sucrose phosphorylase has also been used in a study to investigate the novel transglucosylating reaction with carboxylic compounds.

Biochem/physiol Actions

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (?-D-glucopyranosyl-1,2-?-D-fructofuranoside) and phosphate into D-fructose and ?-D-glucose 1-phosphate.

Enzymatically converts sucrose to D-fructose and D-glucose 1-phosphate.

General description

Sucrose Phosphorylase belongs to glycoside hydrolase, GH13 family. It comprises of four domains with the glucose anomeric carbon-binding site and a glucoside-binding site. The active site residues include Asp192 and Glu232. It is majorly produced by bifidobacteria and lactic acid bacteria. The cross-linked sucrose phosphorylase aggregates is thermostable and could be exploited for industrial catalysis of glycosylation.

Packaging

250, 1000 units in glass bottle

Physical form

Contains sucrose as stabilizer.

Unit Definition

One unit will produce 1.0 µmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.