Ribonuclease T1 from Aspergillus oryzae, ammonium sulfate suspension, 300,000-600,000 units/mg prote

Ribonuclease T1 from Aspergillus oryzae, ammonium sulfate suspension, 300,000-600,000 units/mg prote

Stock Code: 3581251
Manufacturer Part No: R1003-100KU
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Analysis Note


Protein determined by E1%/280


Application


Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .


Biochem/physiol Actions


Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’;-phosphate group of a guanidine ribonucleotide and 5’;-hydroxyl of the adjacent nucleotide. The initial product is a 2’;:3’; cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’;-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.


Physical form


Suspension in 2.8 M (NH4)2SO4 solution


Unit Definition


One unit will produce acid soluble oligonucleotides equivalent to a δA260 of 1.0 in 15 min at pH?7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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