Pyrophosphate reagent, spectrophotometric assay

Stock Code: 3581182
Manufacturer Part No: P7275-1VL
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Application


The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.


Biochem/physiol Actions


The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.


Components


Component ConcentrationImidazole × HCl, pH 7.4 45 mMCitrate 5 mMEDTA 0.10 mMMg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mMβ-NADH 0.8 mMF-6-P 12 mMBovine Serum Albumin 5 mg/mLSugar Stabilizer 5 mg/mLPPi-PFK 0.5 units/mLAldolase 7.5 units/mLGDH 5 units/mLTPI 50 units/mL


Packaging


A technical bulletin is included.


Preparation Note


Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.


Principle


Two moles of NADH are oxidized to NAD+ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.The reagent can be used to detect 10-100 nmoles of pyrophosphate.


Quality


A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and A-glycerophosphate dehydrogenase.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formpowder

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