Phosphorylase b from rabbit muscle, lyophilized powder, >=20 units/mg protein

Phosphorylase b from rabbit muscle, lyophilized powder, >=20 units/mg protein

Stock Code: 3581149
Manufacturer Part No: P6635-25MG
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£345.43 (exc VAT) per 25MG
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Application


The enzyme from Sigma has been used in the calibration of Sepharose C1-6B columns while studying the molecular weight of methylamine dehydrogenase subunits. It has been used in ion mobility-mass spectrometry studies of phosphorylase B ions that have been generated with supercharging reagents, in charge-reducing buffer. It has also been used for the preparation of p32 labeled phosphorylase A using phosphorylase kinase and [32P]ATP.


Phosphorylase b is used to study the conversion mechanism of inactive phosphorylase b to active phosphorylase in muscle. Phosphorylase b is used to study which factors influence the conversion of phosphorylase b to phosphorylase a such as temperature, AMP, fluoride and detergents. It is used to study phosphorylase b deficiency mutations.


Biochem/physiol Actions


Phosphorylase b is a non-active form and is present in resting muscle. Phosphorylase b kinase activity increases significantly when the Mg2+:ATP ratio exceeds 1. The breakdown of ATP during muscle contraction is thought to trigger in vivo conversion of phosphorylase b into a. Phosphorylase b is activated by inosine monophosphate.


Packaging


5 mg in glass bottle


10, 25, 100 mg in poly bottle


Package size based on protein content.


Physical form


Lyophilized powder containing lactose, 5′-AMP, and Mg(OAc)2 (10 µmole per 100 mg protein)


Unit Definition


One unit will form 1.0 µmole of α-D-glucose 1-phosphate from glycogen and orthophosphate in the presence of 5′-AMP, per min at pH 6.8 at 30 °C measured in a system containing phosphoglucomutase, NADP, and glucose 6-phosphate dehydrogenase. (One µmolar unit is equivalent to approx. 45 Cori units.)

Quality Level300
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Impurities~0.01 µmol/mg protein 5′-AMP (This low level will not interfere with phosphorylase and phosphorylase kinase assays.)
Formlyophilized powder

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